AKT1 and central nervous system cancer: MRK-003, alone or combined with GSNO or chloroquine, reduced the baseline side population in primary glioma cultures and suppressed the increase of the side population induced by GSNO [59]; prevented neurosphere formation in HCMV-infected GBM cells and reduced the functionality or number of CSCs [60]; decreased the viability and sphere-formation capacity and increased apoptosis through suppression of the Akt pathway [61]; and induced autophagy in glioma neurosphere lines and reduced cell proliferation, cell growth, and the colony formation ability [62].