Single-cell transcriptomic analysis of genes associated with stemness (OCT4 and SOX2) and EMT (SNAI2, SKP2 and TWIST1) in mouse models of human triple negative breast cancer demonstrated higher expression of these genes in early stage metastatic disease than either in primary tumors or in advanced stage metastatic disease [92]. The gene discussed is POU5F1; the disease is metastatic neoplasm.