EDN1 and triple-A syndrome: Before the time-dependent pathological analysis of AAA wall formation in the hypoperfusion-induced animal model, immunohistochemical analyses of SMemb, neutrophil elastase, angiotensinogen, AT1 receptor, MDA, and ET-1 were performed to elucidate the precise effect of hypoperfusion on the aortic wall on 28 days after the induction of hypoperfusion (Figure 1 and Figure S2).