Despite the strong association of PNPLA3 with human NAFLD/NASH and other liver disease, Pnpla3−/− mice showed no phenotype, including differences in TG hydrolysis, energy/glucose/lipid homoeostasis or hepatic steatosis/injury after high-fat diet (HFD) challenge [10]. This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatotic liver disease.