For this analysis, we included also other human breast cancer cell lines, such as MDA-MB-231 (TNBC), MCF7 (ER+/Her2−) and SK-BR-3 (ER−/Her2+) cells, demonstrating the up-regulation of TENM4 also in the tumorspheres derived from these cell lines. This evidence concerns the gene ERBB2 and breast carcinoma.