Additionally, following 12h of TGF-β1 treatment, expression levels of miR-200b were reduced gradually, implying that miR-200b is a metastasis-inhibiting miRNA in BLCA, which was confirmed when miR-200b overexpression inhibited TGF-β1 induced MMP-16 upregulation and cancer cell migration [42]. Here, MMP16 is linked to bladder transitional cell carcinoma.