Moreover, combined with immunohistochemistry staining (Figure 4B), the semi-quantitative analysis of immunohistochemistry by using Image J (https://imagej.nih.gov/ij/ (accessed on 2 December 2020)) showed that IGF1 and ITGB8 were also upregulated in the lung tissues of IPF patients. The gene discussed is ITGB8; the disease is idiopathic pulmonary fibrosis.