ACE and familial mitral valve prolapse: To ensure that the miRNA signature was specific to MVP and not secondary to other differences between MVP patients and CTRL, we performed a logistic regression associating the model comprising the three miRNAs to the class of interest (MVP vs. CTRL) and to the variables significantly related with the disease (see patient population section); namely, sex, hypertension, angiotensin-converting enzyme (ACE) inhibitors, and beta-blocker therapy.