To this aim, the P53 R175H and R282W proteins were selected (as full-length variants), being hot spot mutations both in sporadic cancers and Li-Fraumeni syndrome; the two mutants are also representative of a loss of function (i.e., R175H) and a partial function (i.e., R282W) mutant, respectively [42,44]. Here, TP53 is linked to Li-Fraumeni syndrome.