The correlation analysis revealed that the expression of many cancer-associated genes such as PGE2S, EGFR, CAV1, NFKB, FOLR1, COX2, BCL2, VEGFA, and POU5F was closely related and proportional to the size of the area of tumor colonies in coculture experiments and that the area of tumor colonies was in negative correlation with CCL2 expression (see Figure 4d, Supplementary Table S3). This evidence concerns the gene NFKB1 and cancer.