Because AES16-2M showed EGF-like effects on skin wound healing in our previous report [16], and because EGF administration reduces the expression of TSLP in skin [18] (an initial factor for AD pathogenesis) [20], AES16-2M was applied to activated normal human epidermal keratinocytes (NHEKs) to evaluate TSLP expression. The gene discussed is EGF; the disease is Alzheimer disease.