The lower limit of detection (LLOD) was determined to be in the range of 5 × 105 NSCLC H1975-sEVs for EGFR, HER2, and IGF-1R, which is below commonly detected sEV amounts in plasma samples from cancer patients and therefore likely sufficient for analyses of sEVs with respect to tumor-associated membrane receptors from various cancers driven by these membrane receptors [36,40]. Here, IGF1R is linked to cancer.