In the KEGG enrichment analysis, while the upregulated genes of glioma grades were enriched in p53, TGF-β, and Notch signaling pathways, prominent downregulated gene clusters fell into synaptic function related pathways, such as glutamatergic, GABAergic, serotonergic, and dopaminergic pathways (Figure 3B, Supplementary Table S3). The gene discussed is TGFB1; the disease is glioma.