PFKFB3 and colorectal carcinoma: To evaluate whether the newly developed PFKFB3 inhibitor [6] (Figure 2A) would be able to reduce PFKFB3 protein expression we decided to treat human umbilical vein endothelial cells (HUVEC) and the CRC cell lines HCT-116, HT-29 and SW-1463 in vitro for 12 h with three different concentrations (10, 25 and 50 μM) of KAN0438757.