Next, to evaluate whether modifications in PFKFB3 protein expression were due to possible changes in its transcription, we incubated HCT-116 and HT-29 cancer cells for 4, 6, 12, 24 and 48 h with 10 and 25 μM KAN0438757 and subsequently performed RT-PCR analyses for PFKFB3 mRNA expression (Figure 2D). Here, PFKFB3 is linked to cancer.