To determine the impact of obesity on myeloid lineage cells in pulmonary metastases, lungs from mice injected with tumor cells were dissociated into single cells, stained to detect CD45, CD11b, Ly6G, Ly6C, and CD115, and myeloid lineage cell populations and GFP+ metastatic cells were analyzed using flow cytometry (Figure S2). The gene discussed is ITGAM; the disease is obesity due to melanocortin 4 receptor deficiency.