To determine the impact of obesity on myeloid lineage cells in pulmonary metastases, lungs from mice injected with tumor cells were dissociated into single cells, stained to detect CD45, CD11b, Ly6G, Ly6C, and CD115, and myeloid lineage cell populations and GFP+ metastatic cells were analyzed using flow cytometry (Figure S2). This evidence concerns the gene CSF1R and obesity due to melanocortin 4 receptor deficiency.