CPS1 was downregulated in HCC tissue when compared to the surrounding non-neoplastic liver tissue (Figure 3b,c, left panel), and was further downregulated in recurrent HCCs and distant HCC metastases (Figure 3b–d), while lymph node metastases displayed a trend towards lower CPS1 expression (Figure 3b,d). This evidence concerns the gene CPS1 and hepatocellular carcinoma.