In addition, CAD expression in HCC tissue correlated with proliferation rate (Ki67, R = 0.37, p = 6.72 × 10−19), alpha fetoprotein (AFP) serum levels (R = 0.32, p = 1.5 × 10−11), AFP immunoreactivity (R = 0.39, p = 3.61 × 10−20), active caspase 3 staining (R = 0.17, p = 7.35 × 10−5), heat-shock protein 70 (HSP70), and glypican 3 (GPC3) immunoreactivity (R = 0.26, p = 1.43 × 10−8 and R = 0.24, p = 1.53 × 10−9). This evidence concerns the gene AFP and hepatocellular carcinoma.