TNF and obesity due to melanocortin 4 receptor deficiency: Figure 2 represents clinical data related to the metabolic risk factors, including TG, HDL-C, LDL-C, CRP, and insulin resistance. Since inflammation is coupled with obesity, inflammatory markers were studied. Leptin and adiponectin, in addition to interleukin (IL)-6, tumor necrosis factor (TNF)-α, and ferritin, were analyzed by ELISA, as well as immunoblotting, followed by densitometry (Figure 1b–f).