For this, M-MDSCs and G-MDSCs isolated from NB-tumor-bearing mice were stimulated with DMSO or ibrutinib for 1 h, followed by the addition of IL6 and GMCSF for 24 h and analysis of mRNA expression of Arg, Ido1, and Tgfβ by qRTPCR. Here, TGFB1 is linked to neuroblastoma.