CD4 and neoplasm: In agreement with the flow cytometry data, immunofluorescence-based analysis of TIL in sections of Formalin-Fixed Paraffin-Embedded (FFPE) tumor tissues (Figure S3A) showed large clusters of CD4+ and CD8+ T cells homogenously distributed throughout the tumor in Vaccine/Bintrafusp/SX-treated tumors and not solely contained to the tumor boundaries.