CD8A and neoplasm: As shown in Figure 4D, flow cytometry analysis of tumors collected at 1 week post-bintrafusp alfa ± vaccine administration (day 21 post-tumor injection) revealed significantly higher frequencies of CD8+ T cells characterized by an effector and effector-memory phenotype (CD8Eff&EM) in the Vaccine/Bintrafusp/SX group compared with the Bintrafusp/SX group or Control tumors.