Intracellular flow cytometry-based analysis of tumor-infiltrating T cells from Vaccine/Bintrafusp/SX-treated mice revealed significantly higher frequencies of proliferative (CD8+ Ki67+) and cytotoxic (CD8+ Granzyme B+) TIL compared to tumors in the Bintrafusp/SX and Control groups (Figure 5A). This evidence concerns the gene MKI67 and neoplasm.