AKT1 and hepatocellular carcinoma: In addition, phosphorylation of protein kinase B (Akt) by binding at Ser473 activates the transcription factor forkhead box protein O1 (FOXO1), which, in turn, inhibits the expression of both PEPCK and G6Pase in high-fat diet (HFD)-fed mice and in hepatocellular carcinoma (HepG2) cells [11].