Therefore, the aims of the present study were: (1) to characterize CSF-derived EVs in healthy subjects and patients with PD, MSA and AP-Tau; (2) to compare EVs profiling in matched CSF and plasma samples of PD and AP patients; (3) to improve our previous diagnostic model, based on plasma-derived EVs by integrating information provided by CSF-derived EVs immunophenotyping. Here, MAPT is linked to multiple system atrophy.