NFKB1 and melanoma: Here, B16F10 melanoma cells were generated to stably express a CMV-driven Dendra2 fluorophore (Dendra2; λex = 409 nm, λem = 507 nm) as well as an NF-κB promoter-driven FLuc reporter (NF-κB-FLuc), where the FLuc bioluminescence magnitude and duration corresponded to the level of NF-κB transcriptional activation (B16F10 NF-κB-FLuc Dendra2).