For example, B16F10 melanoma cells stably expressing a concatenated κB5 promoter-driven FLuc reporter (κB5-FLuc) that also monitors NF-κB activation and a constitutively active IRES-driven green fluorescent protein (GFP) were resolved from each other as well as luminol and L-012 imaging over time during tumor progression (Figure 3a). Here, NFKB1 is linked to melanoma.