Next, we validated that our user-generated pure spectral library correction algorithm could unmix different luciferases that used the same substrate using window chamber tumor-bearing animals injected with two separate B16F10 melanoma tumor cell lines that stably expressed different bioluminescent reporters: one B16F10 cell line stably expressed a ubiquitin promoter-driven CBG reporter and the other B16F10 tumor cell line stably expressed a CMV promoter-driven FLuc reporter. This evidence concerns the gene UBC and melanoma.