B16F10 melanoma cells were generated to stably express dual luciferase–luciferin reporters, the NF-κB promoter-driven FLuc reporter (NF-κB-FLuc) and a constitutively active ubiquitin promoter-driven CBG reporter, both described above (B16F10 NF-κB-FLuc CBG). Here, NFKB1 is linked to melanoma.