Given that scratching evoked by trypsin, which acts on PAR-1, PAR-2, and PAR-4, is suppressed by TRPV1 inhibition or TRPV1 knockout in mice, as well as by the H1-antihistamine cyproheptadine, trypsin-evoked pruritus is considered to be involved in histamine-dependent itch [26]. Here, TRPV1 is linked to Pruritus.