After confirming the Sirt1 and Sirt3 activation on systemic and cardiac insulin resistance, we wanted to know the effect of Sirt1 and Sirt3 activation on lipid parameters i.e., triglycerides, LDL, HDL, serum free fatty acids and total cholesterol in pre-diabetic rats fed with high fructose diet. The gene discussed is SIRT1; the disease is Insulin resistance.