Previous studies have identified numerous risk factors for difficult LC, such as an advanced age, a male sex, the BMI, the American Society of Anesthesiologists physical status score, diabetes, previous abdominal surgery, gallbladder wall thickening, incarcerated stones in the gallbladder neck, fluid retention around the gallbladder, the body temperature, elevated WBCs, the CRP level, a low albumin level, a high bilirubin level, Tokyo Guidelines severity grade II/III AC, and elapsed time before surgery [17,18,19,20,21,22,23,24,25,26,27]. The gene discussed is CRP; the disease is diabetes mellitus.