It has been stated that smear-positive TB and clinically/radiologically severe PTB had a stronger CD8+ T cell response and higher IFN-ɣ values ​​secreted from the TB2 tube, compared with smear-negative TB, EPTB, and LTBI and the addition of peptides to stimulate CD8+ T cells lead to increased sensitivity of the test in detecting latent and active TB infection in patients with destroyed CD4+ T cells [24], [25], [9], [10], [11]. This evidence concerns the gene CD8A and tuberculosis.