As KDM6B was a histone demethylase that mainly mediates H3K27me3 demethylation, to determine whether KDM6B regulates the expression of key genes involved in tumor metastasis by the activity of H3K27 demethylase, we conducted ChIP-Seq (chromatin immunoprecipitation followed by high-throughput sequencing) to characterize the differences in the genomic distribution of H3K27me3 in control and KDM6B-KD OS cells. Here, KDM6B is linked to neoplasm.