Given that EE reversed stroke-induced acetyl-H4 downregulation in the peri-infarct cortex (Figure S2A-C) and the expression and function of GAT-1 are critically regulated by BDNF 17, 33, we performed ChIP assays to investigate the effects of EE on GATs and BDNF genes, including Gat-1, Gat-3, and Bdnf. The gene discussed is SLC6A1; the disease is Stroke.