In view of the role of Wnt5A signaling in actin assembly (16, 24, 27, 28), we studied how the Wnt5A–Actin axis regulates the outcome of infection by a bacterial pathogen (E. coli K1 isolate from a biliary sepsis patient) as compared to a non-pathogen (lab strain E. coli DH5α or E. coli K12-MG1655). Here, WNT5A is linked to infection.