Activation of Wnt5A signaling through added rWnt5A (using PBS as vehicle control), on the other hand, led to significant increase in assembled actin (F-actin) in K1 infected but not K12-MG1655 or DH5α infected RAW264.7 cells during 3 h post infection (T3) (Figure 2C, i and ii), as demonstrated by immunoblotting. The gene discussed is WNT5A; the disease is infection.