Validation of TSPO upregulation in PD rat models and its neurotoxin-induced accumulation in dopaminergic neurons represented the basis for the next in vitro set of analyses, which began by exposing SH-SY5Y cells to 6-OHDA (50 μM, 4–8 h) and measuring TSPO levels via Western blotting (WB) (Fig. 1H, I). This evidence concerns the gene TSPO and Parkinson disease.