While FAP3 was able to reproducibly self-organize into complex three-dimensional colonic structures (> 10 organoids/Matrigel droplet), similar to those of the two WT-hESC lines (H9 and Hues13 > 10 organoids/Matrigel droplet), FAP1 and FAP2-hESCs grew in an unorganized manner and developed only cyst-like structures and cell aggregates of various shape, occasionally with luminal part (4–5 cysts/Matrigel droplet). This evidence concerns the gene NTHL1 and cyst.