TRPV4 and neuropathy: As expression of neuropathy mutants can lead to calcium-mediated cytotoxicity and cell death2,16, and TRPV4 ion channel activity could potentially influence protein–protein interactions, we performed TRPV4–RhoA co-immunoprecipitation experiments in the presence (Fig. 2b–e) or absence (Supplementary Fig. 2a) of TRPV4 ion channel inhibition.