These cells, hereafter referred to as T-Rex-TRPV4WT or T-Rex-TRPV4R269C cells, demonstrate time-dependent TRPV4 expression with appropriate complex glycosylation, cell surface localization, neuropathy mutation-dependent elevation of baseline calcium (Supplementary Fig. 1a–d), and only modest overexpression with 15 ng/ml tetracycline (Supplementary Fig. 1e). This evidence concerns the gene TRPV4 and neuropathy.