In contrast, neuropathy mutant TRPV4 did not inhibit basal RhoA activation (Fig. 4d, e, compare lanes 1 and 2 of T-Rex-TRPV4R269C blot), consistent with a requirement for binding of TRPV4 and RhoA for inhibition of RhoA activation. This evidence concerns the gene TRPV4 and neuropathy.