In ongoing efforts to identify novel TRPV4 protein–protein interactions that are relevant to TRPV4-related neuropathy, we used an unbiased proteomics approach in which we expressed C-terminal FLAG-tagged wild type (WT) or neuropathy mutant (R237L) TRPV4 in HEK293T cells followed by immunoprecipitation and liquid chromatography-mass spectrometry. This evidence concerns the gene TRPV4 and neuropathy.