Neither pharmacologic inhibition of calcium influx with the specific TRPV4 inhibitor HC067047 (HC067) (Fig. 2b, d) nor a pore-inactivating mutation (M680K)39 (Fig. 2c, e) could restore TRPV4–RhoA binding in neuropathy mutants, indicating that disruption of TRPV4–RhoA interactions with neuropathy mutations occurs independent of ion channel activity. The gene discussed is RHOA; the disease is neuropathy.