In addition, mild RhoA inhibition with a low dose of the potent and specific Rho inhibitor exoenzyme C3 transferase56 potentiated the effect of WT TRPV4 on neurite outgrowth and also partially rescued the impaired neurite outgrowth with expression of neuropathy mutant TRPV4 (Fig. 6g, h), suggesting that the failure of neurite outgrowth with neuropathy mutations is at least in part due to excessive RhoA activity. This evidence concerns the gene TRPV4 and neuropathy.