Also, predicted neoepitopes lost due to LOH frequently had higher estimated cancer cell fraction in early disease than those lost through other mechanisms (Supplementary Fig. 12: CAS-A, CAS-B, CAS-C, CAS-D, CAS-E, CAS-F, SK-G, SK-H) and possessed stronger predicted binding affinity to specific HLA alleles in some cases (Supplementary Fig. 13: CAS-D HLA-C:06:02, CAS-F HLA-B:44:03, SK-G HLA-B:07:02 SK-G). The gene discussed is CSN2; the disease is cancer.