PNO1 and central nervous system cancer: PNO1 waschosen for subsequent studies because of its most obvious effect on cell growth.We found that PNO1 was upregulated in glioma cells both in protein and mRNAlevels (Supplementary Fig. S1B, C);lentivirus encoding shRNAs specific against PNO1 were effective, of which,shPNO1-2 was the most effective shRNA (Supplementary Fig. S1D, E).