To further explored the mechanism of PNO1 on glioma, microarrayanalysis between shPNO1 and shCtrl groups of U251 cells was performed andrevealed 1122 DEGs, including 650 downregulated DEGs and 472 upregulated DEGs.KEGG Pathway Enrichment Analysis of these DEGs using Cytoscape indicated thatPNO1 may participate in several functional pathways involved in cellproliferation and metastasis, including focal adhesion and PI3K-Akt signalingpathway (Fig. 5A). The gene discussed is AKT1; the disease is central nervous system cancer.