The data suggested that Gcm1-mediated hyperactivation of Wnt/β-catenin pathway in folate deficiency resembles the signaling of Wnt3a protein in mESCs, as both mechanisms result in inhibition of the expression of anterior neuroectoderm marker Sox1 (Table S2)29. Here, WNT3A is linked to hyperinsulinemic hypoglycemia, familial, 4.