To identify changes in methylation of the TMEFF2 promoter in glioma, we performed bisulfite amplicon sequencing (BSAS) in 1 patient-derived primary glioblastoma cell line (NFHDCD) and 3 glioblastoma cell lines (T98G, LN229, and U87MG), and we also analysed a normal astroglial cell line (SVG p12). This evidence concerns the gene TMEFF2 and glioma.