Smart-seq2-specific HVGs only enriched in two KEGG pathways, while 10X-specific HVGs enriched in 34 pathways, including common pathways in cancer, such as “PI3K–Akt signaling pathway” (Figure S3E), suggesting that HVGs identified by 10X were more conducive to understanding biological difference among samples. This evidence concerns the gene AKT1 and cancer.