As ASM hypertrophy is a feature of asthma (10–12), we investigated whether ORMDL3 expression in ASM influenced hypertrophy by measuring both cell size by FACS and ASM protein to DNA ratio (an index of cell hypertrophy) and by image analysis in ASM derived from hORMDL3Myh11eGFP-cre and WT mice. Here, ORMDL3 is linked to asthma.