The wild type and mutated binding sequences are depicted in Fig. 4C. Subsequently, U937 and HL60 cells were transfected with the miR-4458 mimics or negative control, and the luciferase activity assay results showed that the luciferase activity decreased by 50% in DOCK1-wt and miR-4458 co-transfected AML cells. Here, DOCK1 is linked to acute myeloid leukemia.