To explore the role of GLI1 in the interaction between Hh and the PI3K/AKT pathway in AML cells, we generated both GLI1 OE and KD cell lines from THP-1 and U937 cells by lentiviral transduction and confirmed the changes in GLI1 expression level by qPCR (Fig. S1C–E). This evidence concerns the gene GLI1 and acute myeloid leukemia.