To test the promoting effects of GLI1 on tumor growth in vivo, we injected recipient mice with THP-1/MOCK (as a control group) or THP-1/OE (as experimental group) cells in the flanks of mice (nine mice per group), and their tumor sizes were measured every 2 days from days 12 to 29 (Fig. 4A, B). Here, GLI1 is linked to neoplasm.