RT-PCR for 3′-most exons of kalirin-9 and -12 showed positive results in untreated IMR-32 and SK-N-BE2c (Fig 5H), whereas the full-length kalirin-9 and -12 proteins were barely detectable and a kalirin–GEF2–relevant AB (Fig S5D) did not detect kalirin-8, which implied that other mechanisms (e.g., cleavage by calpains [Miller et al, 2017]) were involved in generating kalirin-8 in NB. This evidence concerns the gene KALRN and neuroblastoma.