FACS assay of orthotopic HCC derived from inoculated Hepa1–6 cells indicated that miR-144/miR-451a overexpression improved the ratios of infiltrating CD8+ T cells and reduced Tregs, but didn’t affect the subsets of B cells, NK/NKT or myeloid-derived suppressor cells (MDSCs) (Fig. S7A-E). Here, CD8A is linked to hepatocellular carcinoma.