In our hNS/PC culture system, we found that K048 and G010 cell lines responded to ZIKV strain PRVABC59 in a similar pattern, both showing significant upregulation of innate immune responses (transcription factors and genes in the TLR3/IFN/MHC-I pathways) and reduced neuronal differentiation (Tuj1+ or MAP2+ cells) during early stage infection (Figs 2 and S1). Here, TLR3 is linked to infection.