When looking into the rate of ZIKV infection, only around 1% cells were infected at the end of differentiation (Day 16), and the majority of ZIKV+ cells were GFAP+ astrocytes with very few Tuj1+ neurons (Fig 3A-3C), indicating differentiated astrocytes may be a potential ZIKV reservoir. This evidence concerns the gene GFAP and Zika virus infectious disease.