To evaluate the functionality of the gH/gL-Plxdc interaction on virus particles both for wildtype RRV and an Eph-binding-negative RRV mutant, we utilized RRV-YFP, an RRV 26–95 strain engineered for constitutive YFP expression upon infection, and RRV-YFP gH-AELAAN, an Eph-binding-negative RRV-YFP mutant that we had previously described (Fig 2A) [23]. The gene discussed is EPHA1; the disease is infection.