EPHA1 and infection: To further analyze the contribution of the gH/gL-Plxdc interaction in the context of infection we constructed virus mutants deleted in the seven amino acid interaction motif in the background of RRV-YFP 26–95 wildtype (RRV-YFP gHΔ21–27), and in the background of an RRV-YFP 26–95 strain mutated in the Eph-interaction motif described previously by our group (RRV-YFP gH-AELAAN, RRV-YFP gHΔ21-27-AELAAN) using a two-step, lambda red-mediated recombination system [35] (Fig 4A).