CD40LG and Zika virus infectious disease: A confirmed case of dengue, chikungunya, and zika was defined as positivity for viral isolation based on non-structural glycoprotein-1 (enzyme-linked immunosorbent assay [ELISA] or rapid test), quantitative reverse transcription polymerase chain reaction (RT-qPCR), or ELISA IgM; RT-qPCR, ELISA IgM, or IgG (ELISA or hemagglutination test); and RT-qPCR or ELISA IgM, respectively.13