EPO and Stroke: Investigation of new treatment strategies for NS should take into account both etiology and timing of injury to be as relevant as possible to human newborn injury and thus translation to the clinic.37 While erythropoietin has demonstrated consistent benefit with post-injury treatment in different models of early brain injury, including stroke,38 we note that multiple doses must be administered and that single-dose therapy does not confer long-term benefits.39 In contrast, our findings demonstrate clear benefits of one-time treatment with SAG following tMCAO.