Compared with those in the splenectomy group, F4/80+ and F4/80+/TGF-β1+ macrophages were significantly increased after rLIGHT treatment (P < 0.05) (Fig. 7b), and worsened liver fibrosis was indicated by Sirius red staining and immunohistochemical staining with a large area of fibrosis after rLIGHT treatment (Fig. 7c). The gene discussed is TGFB1; the disease is Hepatic fibrosis.