CD274 and leukemia: To confirm that aPDL1-CART cells elicit specific cytotoxicity against PD-L1-expressing leukemia cells, aPDL1-CART and control T cells were independently co-cultured, for 24 h and at an E:T ratio of 5:1, with Raji, CA46, and K562, PDL1-Raji, PDL1-CA46, and PDL1-K562 cells.