After dissection, tumor nodules were found in the lymph, liver, spleen, lung and small intestine of mice, which confirmed the successful establishment of DLBCL model. Next, we explored the therapeutic efficacy of CHOP or R-CHOP and combination of GM-CSF against tumor growth by measuring the number and volume of tumor nodules. The gene discussed is DDIT3; the disease is diffuse large B-cell lymphoma.