At a preliminary analysis, 34 sera collected from 27 women within 90 days after infection onset (ten sera in the interval 31–60, and twenty-four in the interval 61–90 days), were HCMV CLIA IgM-negative, as shown in Figure 1A. To be more rigorous, the diagnostic criteria used to define the onset of infection were reviewed in order to restrict the following analysis to women with well-defined infection onset. Here, CD40LG is linked to infection.